ShineProbe® Real Time qPCR MasterMix Kits
Ⅰ、Description
ShineProbe is a 2X convenient premix reagent, specially designed for real time PCR by using various detection probes (eg. TaqMan, Molecular Beacon, etc.) ，but not suitable for sybr green.The master mix contains all reagents needed for qPCR. Only template ,Probe,and PCR primers need to be added by the user. The ROX dye is not provided, For most real-time instruments ROX passive reference dye is not required .This product combines the high performance of Taq, which is an enzyme for hot start PCR utilizing Taq antibody, with a newly developed buffer which provides superior specificity, increased amplification efficiency and high aptitude for high-speed real time PCR. Accordingly, a successful real time PCR is promised with high sensitivity, wide dynamic range and accurate quantification. The reaction chemistry of ShineProbe qPCR Kit is applicable to most real-time PCR instruments, e.g. from Applied Biosystems, Bio-Rad Laboratories, Corbett Research, Roche,and Stratagene.
Ⅱ、Component
ZK00713   50 reactions X 50ul each     Hotstart fluo-PCR mix (2 X conc.)    
ZK00714   100 reactions X 50ul each    Hotstart fluo-PCR mix (2 X conc.)      
Ⅲ、Shipping and Storage

The ShineProbe qPCR Kit is shipped in dry ice or ice bag. Upon arrival, store all kit components at -20°C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20 °C without affecting the performance of the kit. The kit is stable for six months from the date of packaging when stored and handled properly.

Ⅳ、Reaction Setup

Ⅴ、Cycling Protocol

1、Protocol using LightCycler with Taqman probe

   93℃ 2min→93℃5 sec→60℃30 sec

40cyclers

2、Protocol using LightCycler with Molecular Beacon probe

93℃ 2min→93℃5 sec→60℃20 sec→72℃20 sec

40cyclers

3、Protocol using other instruments, e.g. from Applied Biosystems, Bio-Rad Laboratories, Corbett Research, ,and Stratagene. with Taqman probe

94℃ 4min→94℃ 30sec→60℃ 60sec

40cyclers

4、Protocol using other instruments, e.g. from Applied Biosystems, Bio-Rad Laboratories, Corbett Research, ,and Stratagene. with Molecular Beacon probe
                                    94℃ 4min→94℃ 30sec→60℃ 30sec→72℃30sec
                                                                            40cyclers 

　 user manual