大量优惠供应UNG酶

All Shinegene Inc. Products are assayed as needed to ensure performance at the given specifications. Shinegene Inc. certifies this product will perform to the stated specification is handled properly.                         Shanghai ShineGene

Certificate of Analysis     Uracil DNA Glycosylase (UNG)     Applications: Help to eliminate carryover contamination in PCR reactions resulting in fewer false positive result for cloning PCR fragments.   Source: Uracil DNA Glycosylase is the product of the Escherichia coliung gene, and has been cloned, sequenced and expressed in E.coli.   Concentration:1U/ul   Storage Condition: The undiluted enzyme solution is stable when stored at –20℃.   Storage buffer: 50mM Tris-HCL (Ph 8.0),KCL,1mM  EDTA, 1mMDTT, BSA 0.1mg/ml 50%glycerol (v/v).   Unit definition: One unit catalyze the release of 1nmol of free Uracil from 3H-poly(dU) in 1hour at 37℃~50℃.   Unit reaction conditions: 30mM Tris-HCL (Ph 8.3),50mM KCL, 50mM MgCl2,  4ug/ml 3H-poly(dU) , at  7 ╳ 105   cpm/ug,and 1 unit of enzyme in 50 ul for 10min at 37℃~50℃.   Quality control Assays: This product has passed the following quality control assays: functional absence of singleand doble- stranded exonuclease as well as endonuclease and ribbonuclease.     The performance of this product is guaranteed for twelve months from the data of purchase.

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